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Pharmaceutical Processing News
News Release from: Broadley Technologies | Subject: Biomass System
Edited by the Processingtalk Editorial
Team on 06 November 2006
Monitoring density to improve Bioreactor
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Broadley Technologies has been selected by Fogale Nanotech of France as their distributor for the Biomass viable cell density monitoring system in the UK and Ireland
Broadley Technologies has been selected by Fogale Nanotech of France as their distributor for the Biomass viable cell density monitoring system in the UK and Ireland Cell culture and fermentations are used to produce pharmaceutical products or other molecules of interest
This article was originally published on Processingtalk on 31 May 2004 at 8.00am (UK)
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To achieve maximum efficiency or quality assessment during the synthesis of targeted products, on-line information about the active metabolic cell concentration is of crucial importance.
Although the biomass is the catalyst of the bio-production and its concentration is a key parameter in the reaction, the bioreactors are still commonly monitored with only traditional pH, temperature, and dissolved oxygen sensors.
In fact the optical techniques that have been proposed to monitor the biomass suffer from a number of disadvantages such as sensitivity to cell debris and other media components, or loss of linearity.
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From its 20 years of experience in capacitive metrology, Fogale Nanotech has introduced the direct capacitance measurements into a robust, precise and reliable system.
MEASUREMENT PRINCIPLE.
The use of capacitance for on-line process monitoring is very attractive and selective by only taking into account the viable cells, under the influence of an electric field between two electrodes, only cells with intact plasma membranes are polarised and behave as tiny capacitors.
The resulting capacitance (from the Permittivity of the medium) can be measured by a second electrode pair and is accurately and linearly correlated with viable biomass concentration.
Dead cells with broken membranes do not contribute to the signal.
From the Permittivity measurement technique Fogale Nanotech has recently launched the new generation of on-line biomass monitoring tools.
The patented Biomass System is an affordable sensor for viable cell density monitoring, it is directly inserted into the bioreactor with in-situ sterilisable standard 12 mm and 25 mm diameter probes that fit into any standard bioreactor port.
The probes design and materials are ideal for working in a cGMP environment as the wetted materials conform to FDA requirements (PEEK, 316L stainless steel).The Biomass System is ideal for monitoring animal cell in suspension (including clumping cell lines) or attached to microcarriers, it is also routinely used for yeast or bacteria batch and fed-batch processes with high biomass concentration up to 200g/l dry weight.
It includes a unique triple frequency measurement that results in insensitivity to cell size variations and high accuracy in the signal.
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