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Food Processing News
News Release from: Metrohm UK | Subject: MISP
Edited by the Processingtalk Editorial
Team on 02 December 2005
Carbohydrate determination using IC
New ion analysis method from Metrohm to determine carbohydrates in complex food samples with minimal sample handling uses automated Ion Chromatography
Food is a complex sample matrix that historically has contained many different manual sample preparation steps that were both labour intensive and time consuming to the user With Metrohm and their novel MISP (Metrohm Inline Sample Preparation) Ion Analysis technology it is possible to alleviate most of the manual sample preparation and reduce the errors associated from sample handling
This article was originally published on Processingtalk on 26 Jul 2007 at 8.00am (UK)
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The Metrohm Inline Sample Preparation (MISP) for ion chromatography allows the determination of iodide in milk: the matrix of milk is removed by inline dialysis prior to sample injection
In-line sample preparation for ion chromatography
Crystal clear, clinically pure, no bacteria and no particles: this would be an ideal sample for ion chromatography: but normal samples require thorough sample preparation
Typically sample pre-treatment for different solid food samples was maceration with heat to form a pulp, then addition of water to bulk up the solution, allow to stand, collect the top portion before a large dilution (to reduce the analytes from percent to milligram per litre range) and finally filtration to remove the particles that would severely reduce the lifetime and impair the operation of the separation columns required for the analysis of ions using chromatography.
The analysis of different carbohydrates in food samples (fish products in this application) can reliably and precisely be performed using Ion Chromatography (IC) using the 817 Bioscan from Metrohm with a 818 Advanced IC Pump and 812 Valve Unit in tandem with the 838 Advanced IC sample processor equipped with unique patented sample handling technology.
All Ion Chromatography instruments from Metrohm come backed by the COOL guarantee (Cost Of Ownership is Lower than with competitor instruments) for complete piece of mind, in addition to the proven Metrohm reputation in Ion Analysis from sixty years of experience and expertise.
Further reading
Sugar determination in Dairy products
In food chemistry the determination of sugars in dairy products is of the utmost importance: dialysis improves inline analysis
Tighter process control in acid pickling baths
Online ion chromatography from Metrohm has enabled tighter process control of acid pickling baths, as the final cleaning stage prior to metal plating
The food sample was macerated and 50mls of de-ionised water was added, allowed to stand and the supernatant portion used in the autosampler vial.
From this point the analysis was fully automated resulting in improved accuracy, reproducibility and a reduction in running costs.
A portion of the sample was then diluted with water with air mixing (to aid homogeneity) using the time proven 800 Dosino liquid handling device, before dialysis through the novel and patented Metrohm dialysis cell equipped on the side of the 838 Advanced IC Sample Processor to remove any minute particles.
10micro-litres of the dialysed sample was injected into the IC system for analysis and a typical chromatogram obtained showing a presence of glucose, fructose, sucrose and maltose in the sample.
To learn more about carbohydrate applications and how Metrohm Inline Sample Preparation technology can reduce the amount of human intervention and handling to free up laboratory time with the additional benefit of reduced operating costs, then call our technical service line where our friendly team of specialists will be pleased to discuss your project requirements in further detail. Request a free brochure from Metrohm UK ...
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