Product category:
Liquid Analysis: Titration, HPLC, IC
News Release from: Thermo Fisher Scientific | Subject: Hypercarb Columns
Edited by the Processingtalk Editorial
Team on 23 August 2004
Columns exploit high-temperature
chromatography
Thermo Electron Corp has a new range of HPLC applications at ultra-high temperatures using Hypercarbu (porous graphitic carbon) columns.
Thermo Electron Corp has a new range of HPLC applications at ultra-high temperatures using Hypercarbu (porous graphitic carbon) columns The use of ultrahigh temperatures (up to 200C) in reversed phase liquid chromatography (RP-HPLC) has several advantages
This article was originally published on Processingtalk on 11 Aug 2008 at 8.00am (UK)
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Higher peak capacity - at higher temperatures solvent viscosity is reduced, which enhances the mass-transfer between mobile and stationary phases resulting in higher efficiencies, providing sharper peaks and increased peak capacity.
Higher sensitivity - because more efficient peaks and shaper peaks are obtained at high temperatures, there is an improvement in peak height, therefore better signal-to-noise ratio.
Higher speed - at high temperatures backpressure is reduced and so higher flow rates can be used for fast separations without compromising efficiency.
Columns packed with modified silicas, which are generally used in RP-HPLC, should not be used above 60-80C.
At these extreme temperatures, hydrolysis of the organosilane bond or dissolution of the silica may occur.
Hypercarb is the ideal stationary phase for high temperature HPLC, as it is not affected by physical or chemical degradation at high temperature regardless of mobile phase used.
Hypercarb is 100% carbon, which is chemically very stable and robust, and therefore can be routinely used up to 200C under either isothermal or temperature gradient conditions.
Thermo's new applications illustrate the use of temperature as a method development tool for the chromatographer in the environmental, pharmaceutical and other analytical laboratories.
They show how high temperatures or temperature gradients can be used to achieve fast, high efficiency separations.
The use of a mobile phase of pure water, combined with temperature gradients, is also demonstrated for the separation of very polar analytes on Hypercarb columns.
Hypercarb columns are available packed with 3, 5 and 7um particle sizes and in a range of hardware designs, internal diameters and lengths.
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