Real time monitoring of enzyme catalysed reactions

A new technical presentation discussing the use of HR-US technology for real time monitoring of enzyme catalyzed reactions was given by Ultrasonic Scientific at the recent PITTCON 2006 conference

A new technical presentation discussing the use of HR-US technology for real time monitoring of enzyme catalyzed reactions was given by Ultrasonic Scientific, innovator in High-Resolution Ultrasonic Spectroscopy (HR-US), at the recent PITTCON 2006 conference.

HR-US is a powerful, non-destructive technique suitable for a wide range of enzyme controlled processes in the food, chemical, environmental and biotechnology industries.

It allows the direct, real-time detection of enzymatic reactions, chemical reactions, micro-structural transitions, gel network formation, hydrolysis of proteins, evolution of particle size and the determination of enzyme activity.

Enzymes are important because they accelerate reactions, act only on specific substrates, are safe and easy to control, can replace harsh chemicals and are biodegradable.

Analytical instrumentation for the analysis of enzyme reactions has traditionally been dominated by spectroscopic technologies including electromagnetic spectroscopy such as UV, IR, NMR, FTIR and fluorescence, all of which present considerable downfalls.

In the new presentation, Ultrasonic Scientific demonstrates the use of its award-winning range of HR-US spectrometers and their accompanying accessories to conduct a series of analyses for different enzyme catalysed reactions monitoring applications.

The applications demonstrated in the poster include.

* Hydrolysis of Z-GLGGA peptide by proteinase K.

* Proteolysis of BSA.

* Hydrolysis of casein by proteinase K.

* HR-US measurements in rennet milk.

* Hydrolysis of cellulose.

* Hydrolysis of maltodextrin.

* Detection of glucose content in blood.

* Hydrolysis of peroxide by catalase.

HR-US is an innovative award-winning technique based on the precision measurements of velocity and attenuation of the ultrasonic waves propagating through the analysed sample.

The technique is extremely sensitive, fast, cost-effective and can be used in concentrated and opaque samples directly.

It requires small sample volume (to 0.03 ml), no markers, no dilution and minimum or no sample preparation.

Finally it involves no special requirements such as optical transparency, optical activity or electrical conductance for the characterisation of samples.

The new technical presentation is available direct from Ultrasonic Scientific.

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