Product category:
Liquid Analysis: Spectroscopy, Colour
News Release from: Wyatt Technology | Subject: MALS
Edited by the Processingtalk Editorial
Team on 12 March 2007
MALS instruments help malaria research
A newly published application note shows how the novel Wyatt Technology Multi-Angle Light Scattering (MALS) instruments can characterise novel malaria vaccine candidates
Wyatt Technology, the world leader in absolute macromolecular characterisation products, has been chosen by the Malaria Vaccine Development Branch (MVDB) of the National Institute of Allergy and Infectious Diseases (NIAID) for its instruments to characterise the recombinant protein vaccines it produces The Wyatt novel Multi-Angle Light Scattering (MALS) instruments were found to facilitate the MVDB understanding of purity, identity and aggregation states of recombinant protein vaccine candidates after they were separated by Size Exclusion Chromatography (SEC)
This article was originally published on Processingtalk on 18 Jun 2007 at 8.00am (UK)
Related stories
MALS in use monitoring protein aggregation
A new application note demonstrates the ability to monitor protein aggregation states in different buffers using the Wyatt Technology light scattering detectors
Monitoring sodium hyaluronate concentrations
New application note from Wyatt demonstrates the accurate and precise determination of molecular weight and concentration of sodium hyaluronate in mobile phase solution and mixed with gelatine
Malaria remains one of the world's deadliest diseases for people living in the tropics, particularly in Africa.
Globally, malaria causes more than 1 million deaths each year with approximately 60% of these deaths occurring in the poorest 20% of the total global population.
Malaria kills one child under five years of age every 30 seconds.
Further reading
Automated molecular characterisation in Zimm plots
Wyatt Technology DAWN Multi-Angle Light Scattering (MALS) and Optilab rEX (refractive index detector) achieve rapid, automated molecular characterisation via a Zimm plot experiment
Instruments make absolute particle sizing easy
Two new instruments from Wyatt Technology - the Eclipse and the Dawn EOS - make absolute particle sizing an accessible alternative to traditional liquid chromatography methods.
Unfortunately, malaria parasites have developed a variety of mechanisms to evade host immune responses, thus making the development of a successful vaccine very challenging.
The Malaria Vaccine Development Branch of NIAID was established in 2001 at the Institute's Rockville, Maryland research facility.
MVDB is an 8,000-square-foot, state-of-the-art biotechnology laboratory designed to develop, produce and characterise pilot lots of promising malaria vaccine candidates based on recombinant proteins.
Those vaccine candidates are intended for use in Phase I and II human trials.
For this particular application, the solution profile of a candidate antigen was analysed using analytical SEC with on-line MALS, Refractive Index (RI) and UltraViolet (UV) detection.
A high performance liquid chromatograph (HPLC) and a PhotoDiode-Array PDA detector were connected in series to Wyatt Dawn EOS and Optilab RI detectors run by Wyatt Astra software suite.
An isocratic elution at 0.5 mL/min was performed using a TSK gel G3000PWxl column and a TSK gel Guard PWxl column with 0.04mM KH2PO4, 2.97mM Na2HPO4*7H2O, 308mM NaCl, 0.5M urea, pH 7.4, 0.02% sodium azide for mobile phase.
The SEC-MALS results clearly demonstrated that the solution state of the vaccine candidate was comprised of three populations, namely monomer, dimer and tetramer.
This fact was later verified by boundary sedimentation equilibrium studies.
A copy of the new application note, entitled "Novel Malaria Vaccine Candidates" is available from Wyatt.
For more information on the Wyatt Technology SEC and MALS instruments and its Astra V software solution or to obtain a copy of this application note, please visit the website, or contact Wyatt direct.
• Wyatt Technology: contact details and other news
• Email this article to a colleague
• Register for the free Processingtalk email newsletter
• Processingtalk Home Page
Search the Pro-Talk network of sites
